Edit: It's called Ambush, not Assault, sorry đ
When I first started using the Blood Katana, I was a little confused by the description of the Ambush Skill, stating "If it hits an enemy with Booster, it can be used once more." With some testing, I understand now that this means enemies that are suffering from certain negative effects such as Bleeding, Fire, and Electricity, but the use of the word booster, as well as booster's tool-tip definition caused some initial confusion for me. Boosters are given to us the player as positive effects such as the booster offered at the beginning of a run near Isaac, the boosters that activate at the beginning of combat that are granted by some Modules, and the booster from the hot chocolate at that one ? room.
Because of how the game trains the player to think about the keyword Booster, it was confusing at first when Ambush's additional strikes weren't possible on enemies that have any icon over their head such as Administrator Wolves. Even the page for Booster in the Log describes them as granting "powerful effects," and there is an entirely different page for Status Effects.
After looking through all of that, it seems to me that the Ambush Skill should instead read as: "If it hits an enemy with a Status Effect, it can be used once more."